Journal: Scientific Reports

Article Title: A tool kit for rapid cloning and expression of recombinant antibodies

doi: 10.1038/srep05885

Figure Lengend Snippet: (A–B) Flow cytometric histograms showing fluorescent intensity of cells incubated with purified IgG/IgE antibodies plus secondary FITC-labelled goat anti-human IgG/IgE (open) and cells with secondary antibody only (filled) against the number of cells. (A) Flow cytometric assessment of anti-CSPG4 IgG 1 /IgE antibodies shows specific binding to native CSPG4 antigen present on the cell surface of A375 melanoma cells and no binding above background to primary melanocytes. (B) Fc regions of anti-CSPG4 IgG 1 and 102.1F10 IgG 4 isotypes demonstrate effector-binding to U937 monocytic cell line, expressing human Fcγ receptors. The IgE antibody isotypes also bind similarly to RBL SX38 mast cells, expressing human FcεR1 receptor. (C) Immunofluorescence staining of A375 cells confirms specific binding of anti-CSPG4 IgG 1 /IgE and no background binding with isotype control hapten specific NIP-IgG 1 /IgE detected by goat anti-human IgG/IgE-FITC. (D) Grass pollen allergen specificity is confirmed by anti-human sandwich ELISA, showing specific binding of recombinant 102.1F10 IgG 4 /IgE and original patient serum to the ELISA plate-bound Phl p 7 allergen and no binding above background with unspecific human myeloma IgG 4 and MOv18 IgE isotype controls.

Article Snippet: Primary human epidermal melanocytes (PCS-200-012, ATCC) were grownin Dermal Cell Basal Medium (PCS-200-030, ATCC) and supplemented with the Melanocyte Growth Kit (PCS-200-041, ATCC).

Techniques: Incubation, Purification, Binding Assay, Expressing, Immunofluorescence, Staining, Control, Sandwich ELISA, Recombinant, Enzyme-linked Immunosorbent Assay

Journal: Clinical, Cosmetic and Investigational Dermatology

Article Title: LncRNA LNCOC1 is Upregulated in Melanoma and Serves as a Potential Regulatory Target of miR-124 to Suppress Cancer Cell Invasion and Migration

doi: 10.2147/CCID.S359786

Figure Lengend Snippet: The expression levels of LNCOC1 were increased in melanoma cell lines and enhanced cell proliferation of primary human melanocytes. Expression of LNCOC1 and miR-124 in primary human melanocytes and melanoma cell lines were detected by RT-qPCR. The expression levels of LNCOC1 were much higher in SK-MEL-3 and A375 than that in primary melanocytes ( A and B ). Overexpressed LNCOC1 could promote the proliferation of primary melanocytes. As a suppressive factor in cancer, miR-124 had an inhibitory effect on melanocytes proliferation ( C ). * p < 0.05.

Article Snippet: Primary neonatal normal human epidermal melanocytes were obtained from Sangon Biotech (Shanghai, China).

Techniques: Expressing, Quantitative RT-PCR

Journal: Clinical, Cosmetic and Investigational Dermatology

Article Title: LncRNA LNCOC1 is Upregulated in Melanoma and Serves as a Potential Regulatory Target of miR-124 to Suppress Cancer Cell Invasion and Migration

doi: 10.2147/CCID.S359786

Figure Lengend Snippet: The scheme of mechanism of the effect of LNCOC1 and miR-124 on melanoma. In MM patients, LNCOC1 displayed an elevated level in their tumor tissues. Also, a decrease in miR-124 was found in those tumor samples, which might contribute to the poor survival of MM patients. On the other hand, overexpression of miR-124 could downregulate LNCOC1 expression, suggesting LNCOC1 is an inhibitory target of miR-124 to suppress the invasion and migration of melanoma cells. In addition, LNCOC1 reduction and miR-124 elevation inhibited primary human melanocyte proliferation to play protective roles in the progression of melanoma.

Article Snippet: Primary neonatal normal human epidermal melanocytes were obtained from Sangon Biotech (Shanghai, China).

Techniques: Over Expression, Expressing, Migration